Evaluation of an alternative confirmatory strategy for the diagnosis of HIV infection in Dar Es Salaam, Tanzania, based on simple rapid assays.

摘要: Abstract Alternative confirmatory strategies for detection of antibodies to HIV using enzyme-linked immunosorbent assays (ELISAs) have been shown be useful in laboratories with limited resources. Three simple and rapid antibody (Capillus, Serocard Determine) were evaluated 1412 fresh serum samples order formulate an alternative strategy the diagnosis infection. All sera also tested by anti-HIV ELISA all reactive any a second as well Western blot. hundred eighty-three found HIV-1 positive, while 1017 negative; 12 which one or more had indeterminate blot results these considered seronegative during analysis. sensitivity 100%. The initial specificity 98.7, 98.2 97.9% Capillus, Determine, respectively. In use Capillus followed Determine gave 99.9 99.8%, 99.3%. A testing 100% (95% CI; 99.6–100%) could achieved sequential three simple/rapid repeat Serocard.