Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes.
作者： Nancy L Kedersha , Marie-Christine Miquel , Dennis Bittner , Leonard H Rome , None
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摘要: Vaults are cytoplasmic ribonucleoprotein structures that display a complex morphology reminiscent of the multiple arches which form cathedral vaults, hence their name. Previous studies on rat liver vaults (Kedersha, N. L., and L. H. Rome. 1986. J. Cell Biol. 103:699-709) have established composition is unlike any known class RNA-containing particles in they contain copies unique small RNA more than 50 single polypeptide 104,000 Mr. We now report isolation from numerous species show appear to be ubiquitous among eukaryotes, including mammals, amphibians (Rana catesbeiana Xenopus laevis), avians (Gallus Gallus), lower eukaryote Dictyostelium discoideum. Electron microscopy reveals purified these diverse similar both dimensions morphology. The various also composition; each being composed major with an approximate mass 100 kD several minor polypeptides molecular masses those seen rat. Antibodies raised against recognize vault protein all Dictyostelium. therefore strongly conserved broadly distributed, suggesting function essential eukaryotic cells.
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Maurice Sussman, Chapter 2 Cultivation and Synchronous Morphogenesis of Dictyostelium under Controlled Experimental Conditions Methods in Cell Biology. ,vol. 28, pp. 9- 29 ,(1987) , 10.1016/S0091-679X(08)61635-0
Carl R. Merril, David Goldman, Margaret L. Van Keuren, [17] Silver staining methods for polyacrylamide gel electrophoresis Methods in Enzymology. ,vol. 96, pp. 230- 239 ,(1983) , 10.1016/S0076-6879(83)96021-4
Hugh J.L. Fryer, George E. Davis, Marston Manthorpe, Silvio Varon, Lowry protein assay using an automatic microtiter plate spectrophotometer Analytical Biochemistry. ,vol. 153, pp. 262- 266 ,(1986) , 10.1016/0003-2697(86)90090-4
William F. Loomis, Dictyostelium Discoideum: A Developmental System ,(1975)
J. E. Darnell, W. F. Doolittle, Speculations on the early course of evolution Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 1271- 1275 ,(1986) , 10.1073/PNAS.83.5.1271
Max L. Birnstiel, Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles ,(1988)
Peter Walter, Vishwanath R. Lingappa, Mechanism of protein translocation across the endoplasmic reticulum membrane. Annual Review of Cell Biology. ,vol. 2, pp. 499- 516 ,(1986) , 10.1146/ANNUREV.CB.02.110186.002435
Nancy L Kedersha, Leonard H Rome, None, Preparative agarose gel electrophoresis for the purification of small organelles and particles. Analytical Biochemistry. ,vol. 156, pp. 161- 170 ,(1986) , 10.1016/0003-2697(86)90168-5
S. G. Harris, S. O. Hoch, H. C. Smith, Chemical cross-linking of Sm and RNP antigenic proteins. Biochemistry. ,vol. 27, pp. 4595- 4600 ,(1988) , 10.1021/BI00413A002
Alan M. Weiner, Eukaryotic nuclear telomeres: Molecular fossils of the RNP world? Cell. ,vol. 52, pp. 155- 157 ,(1988) , 10.1016/0092-8674(88)90501-6