CDEBP, a site-specific DNA-binding protein of the 'APP-like' family, is required during the early development of the mouse.

摘要: A murine protein, termed CDEBP, was previously shown to bind the double-stranded DNA motif GTCACATG, identical yeast centromeric element CDEI. The cDNA sequence showed three domains with extensive similarities amyloid beta precursor protein (APP). is homologous over its entire length human designated APPH. In situ immunofluorescence assays using antibodies raised against distinct parts of CDEBP detected discrete sites accumulation inside interphase nucleus, and bulk not associated mitotic chromosomes. One complexes CDEI oligonucleotides by gel shift assay present when had been selectively removed from nuclear extracts immunoprecipitation. We reported that microinjection into one-cell mouse embryos fragments including results in an early arrest development abnormal nuclei containing variable amounts DNA. same characteristic figures were observed treated antisense complementary coding region. Complexes between genome thus appear play a critical role replication/segregation embryonic genome.