Defining conditions for the efficient in vitro cross-linking of proteins to DNA by chromium(III) compounds.

摘要: The formation of cross-links between bovine serum albumin and DNA in the presence chromium(III) chloride was found to be highly pH dependent. In vitro, such lesions were only formed at acidic values pH, but not detected neutral pH. Complexes GSH/GSSG similarly failed induce DNA-protein physiological Our findings indicate that generated vitro may directly relevant observed cultured cells a physiologically model for efficient cross-linking proteins has yet devised.