Development of extender based on soybean lecithin for its application in liquid ram semen.
作者： P. de Paz , M.C. Esteso , M. Alvarez , M. Mata , C.A. Chamorro
DOI: 10.1016/J.THERIOGENOLOGY.2010.03.022
关键词:
摘要: The soybean lecithin is used as a phospholipids source for the commercial extenders available freezing bull semen which allows replacing traditional membrane protective of animal origin (egg yolk). These have been tested in various livestock species but specific adjustments cannot be made due to trade protection. aim present study was develop soybean-based extender analyzing optimal conditions preparation, handling, and storage order optimize its use liquid ram semen. Its effect on quality also studied. Different TES-Tris-Fructose-based were prepared using two types (S20 S95) differentiated by their lipid composition (complex or simple, respectively). up temperatures: 20 °C (PT20) 37 (PT37); centrifuged filtered at stored 15 5 (ST15 ST05) several periods (from 6 hours 7 days). Three different concentrations (0.5%, 2%, 3.5%) evaluated each extender. amount nature high performance chromatography (HPLC) method according parameters applied preparation. In general, highest quantity observed S20 Centrifugation-filtration process during preparation reduces 50% medium experiments. not affected significantly temperature Storage affects with minimum values °C. As time, both showed stable course 2 days obtained higher concentration (3.5%) content under tested. Finally, sperm motility viability new analyzed. We that Sperm S20-2% control (UL). Our results suggest from °C, filtered, preserve an egg-yolk
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