Analysis of a sunflower polyubiquitin promoter by transient expression
作者： Marie-Noelle Binet , Marc Lepetit , Jacques-Henry Weil , Luc-Henri Tessier
DOI: 10.1016/0168-9452(91)90073-H
关键词:
摘要: Abstract We have recently isolated two sunflower polyubiquitin genes (UbB1 and UbB2) which are induced by heat-stress show a highly conserved region of about 250 bp upstream the transcription initiation site. The promoter activity this has been analyzed transient expression using β-glucoronidase (GUS) gene as reporter in tobacco protoplasts. GUS absence any external inducer is five times higher than that observed under control CaMV 35S promoter. Analysis different deletions within 250-bp showed 65-bp sequence responsible for most This intron-independent not enhanced when protoplasts heat-stressed. Sequence homologies strongly suggest UbB1 plays major role stress response sunflower.
elsevier.com
sci-hub.se 参考文章(23)
Ronald F. Hough, Gregory W. Pratt, Martin Rechsteiner, Ubiquitin/ATP-Dependent Protease Springer US. pp. 101- 134 ,(1988) , 10.1007/978-1-4899-2049-2_5
Avram Hershko, Aaron Ciechanover, The ubiquitin pathway for the degradation of intracellular proteins. Progress in Nucleic Acid Research and Molecular Biology. ,vol. 33, pp. 19- 56 ,(1986) , 10.1016/S0079-6603(08)60019-7
Daniel Finley, Alexander Varshavsky, The ubiquitin system: functions and mechanisms Trends in Biochemical Sciences. ,vol. 10, pp. 343- 347 ,(1985) , 10.1016/0968-0004(85)90108-2
J Callis, M Fromm, V Walbot, Introns increase gene expression in cultured maize cells. Genes & Development. ,vol. 1, pp. 1183- 1200 ,(1987) , 10.1101/GAD.1.10.1183
H.Joseph Yost, Robert B. Petersen, Susan Linquist, RNA metabolism: strategies for regulation in the heat shock response Trends in Genetics. ,vol. 6, pp. 223- 227 ,(1990) , 10.1016/0168-9525(90)90183-7
Richard A. Jefferson, Assaying chimeric genes in plants: The GUS gene fusion system Plant Molecular Biology Reporter. ,vol. 5, pp. 387- 405 ,(1987) , 10.1007/BF02667740
P. MALIGA, ÁGNES SZ.-BREZNOVITS, L. MÁRTON, Streptomycin-resistant Plants from Callus Culture of Haploid Tobacco Nature. ,vol. 244, pp. 29- 30 ,(1973) , 10.1038/NEWBIO244029A0
Richard D. Vierstra, Ubiquitin, a key component in the degradation of plant proteins Physiologia Plantarum. ,vol. 70, pp. 103- 106 ,(1987) , 10.1111/J.1399-3054.1987.TB08704.X
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
Ursula M. Pfitzner, Artur J. P. Pfitzner, Howard M. Goodman, DNA sequence analysis of a PR-1a gene from tobacco: Molecular relationship of heat shock and pathogen responses in plants Molecular and General Genetics MGG. ,vol. 211, pp. 290- 295 ,(1988) , 10.1007/BF00330606