Automated High-Throughput Fatty Acid Analysis of Umbilical Cord Serum and Application to an Epidemiological Study

摘要: Large population studies show that polyunsaturated fatty acids are important for human health, but determining relationships between the health benefits and acid content has been hampered by unavailability of labor-effective high-throughput technologies. An automated high throughput analysis was developed from a previous procedure based on direct transesterification including automation chemical procedures, data acquisition automatic processing. The method validated applied to umbilical cord serum samples in an epidemiological study. linear range 1-600 μg/mL with r² ≥ 0.99. within-run CV <5.4% 23 recoveries over three concentrations were 76-119% low-lipid matrix exception 14:0. concentration as measured robotic plasma good agreement Lepage & Roy method. profile American subjects (n = 287) showed average 38.0, 24.9, 32.0 4.6% total saturates, monounsaturates, n-6 n-3 polyunsaturates, respectively. This is first report complete, validated, cost-effective, automated, measurement along application population-based Automated coupled processing greatly facilitates throughput, 72 transesterified 6 h, required large studies.