作者: Robin Jonas , Andreas Klusch , Martin Schmelz , Marlen Petersen , Richard W. Carr
DOI: 10.1371/JOURNAL.PONE.0139107
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摘要: Nine isoforms of voltage-gated sodium channels (NaV) have been characterized and in excitable tissues they are responsible for the initiation conduction action potentials. For primary afferent neurons residing dorsal root ganglia (DRG), individual may express multiple NaV extending neuron's functional capabilities. Since expression can be differentially regulated by neurotrophic factors we examined consequences exposure to either nerve growth factor (NGF) or glial cell line-derived (GDNF) on potential outgrowing cultured porcine neurites DRG neurons. Calcium signals were recorded using exogenous intensity based calcium indicator Fluo-8®, AM. In 94 neurons, conducted along response electrical stimulation soma. At an image acquisition rate 25 Hz it was possible discern transients stimuli. The peak amplitude electrically-evoked limited ability neuron follow stimulus frequency. frequency required evoke a half-maximal approximately 3 at room temperature. 13 14 (93%) NGF-responsive neurites, TTX-r alone sufficient support propagated signals. contrast, mediated NaVs evident only 4 11 (36%) from somata GDNF. This establishes basis assessing signaling imaging techniques suggests that, pig, nociceptor classes relying properties isoforms, such as cold-nociceptors, most probably derive