Electrical approach to study rhodopsin activation in single cells with early receptor current assay.

摘要: The ERC is a conformation-dependent charge motion similar to the gating currents of ionic channels. Both waveforms and bandwidth ERCs channel are similar, providing support initial suggestion that ERP was kind current. In channels electrostatic field promotes alpha-helical elements stimulate large-scale molecular events promote opening pore. expressed mutants has contributed significantly understanding mechanism activation. Given known role electrical processes rhodopsin activation, approach applied mutant wild-type visual pigments likely lead fuller conformational This method currently well suited investigate later phases activation thought be in nature. We anticipate studies will make significant contributions how breakdown interaction between PSB its counterion initiated propagated induce proton uptake on cytoplasmic surface pigment shaping transducin docking domain. encourage collaboration apply methodology interesting retinal analogs. expect can soon understand rapid displacements associated with photochemistry (i.e., R1), effects transduction proteins R2, measurement during cone