Decreasing calreticulin expression lowers the Ca2+ response to bradykinin and increases sensitivity to ionomycin in NG-108-15 cells.

摘要: It has been suggested that the multifunctional protein, calreticulin, is a major calcium sequestering protein in inositol 1,4,5-trisphosphate receptor-containing endoplasmic reticulum subcompartment. In neuroblastoma X glioma NG-108-15 cells, bradykinin can effectively stimulate release of and cause cytosolic transient. To explore function calreticulin as an intracellular we investigated dynamics cells after treatment with antisense oligonucleotide against CrtAS1. Cells treated either CrtAS1 or corresponding sense CrtPS1 were examined for their content by Western blotting, amplitude transient response to bradykinin, sensitivity toward ionophore, ionomycin. Treatment decreased amount comparison CrtPS1-treated untreated control cells. At same time, CrtAS1-treated had significantly reduced more sensitive ionomycin-induced cell death. These data show level expression directly related storage capacity 1,4,5-trisphosphate-sensitive pool indicate direct relationship between protection cytotoxic overload.