In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation.

作者: Kazuchika Miyoshi , Keisuke Sato , Mitsutoshi Yoshida

DOI: 10.1089/CLO.2006.8.159

关键词:

摘要: The present study was carried out to examine the activation and development of cloned embryos produced by transferring miniature pig somatic cells into enucleated farm oocytes after exposing ultrasound. rates pronucleus-like structure formation polar body-like extrusion in exposed ultrasound did not differ from those applied electric pulses. Although there no significant difference blastocyst between different methods, mean number blastocysts developed activated significantly (p < 0.05) higher than that obtained applying results showed stimulation can induce vitro derived cells.

参考文章(30)
T. Wakayama, A. C. F. Perry, M. Zuccotti, K. R. Johnson, R. Yanagimachi, Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei Nature. ,vol. 394, pp. 369- 374 ,(1998) , 10.1038/28615
K. J. McCreath, J. Howcroft, K. H. S. Campbell, A. Colman, A. E. Schnieke, A.J. Kind, Production of gene-targeted sheep by nuclear transfer from cultured somatic cells Nature. ,vol. 405, pp. 1066- 1069 ,(2000) , 10.1038/35016604
Irina A. Polejaeva, Shu-Hung Chen, Todd D. Vaught, Raymond L. Page, June Mullins, Suyapa Ball, Yifan Dai, Jeremy Boone, Shawn Walker, David L. Ayares, Alan Colman, Keith H. S. Campbell, Cloned pigs produced by nuclear transfer from adult somatic cells Nature. ,vol. 407, pp. 86- 90 ,(2000) , 10.1038/35024082
Akira Onishi, Masaki Iwamoto, Tomiji Akita, Satoshi Mikawa, Kumiko Takeda, Takashi Awata, Hirohumi Hanada, Anthony CF Perry, Pig cloning by microinjection of fetal fibroblast nuclei Science. ,vol. 289, pp. 1188- 1190 ,(2000) , 10.1126/SCIENCE.289.5482.1188
EVAN C. UNGER, THOMAS P. McCREERY, ROBERT H. SWEITZER, ULTRASOUND ENHANCES GENE EXPRESSION OF LIPOSOMAL TRANSFECTION Investigative Radiology. ,vol. 32, pp. 723- 727 ,(1997) , 10.1097/00004424-199712000-00001
Philippe Collas, Rafael Fissore, James M. Robl, Eddie J. Sullivan, Frank L. Barnes, Electrically induced calcium elevation, activation, and parthenogenetic development of bovine oocytes. Molecular Reproduction and Development. ,vol. 34, pp. 212- 223 ,(1993) , 10.1002/MRD.1080340214
Keisuke Sato, Mitsutoshi Yoshida, Kazuchika Miyoshi, Utility of ultrasound stimulation for activation of pig oocytes matured in vitro. Molecular Reproduction and Development. ,vol. 72, pp. 396- 403 ,(2005) , 10.1002/MRD.20352
R.S Prather, R.J Hawley, D.B Carter, L Lai, J.L Greenstein, Transgenic swine for biomedicine and agriculture Theriogenology. ,vol. 59, pp. 115- 123 ,(2003) , 10.1016/S0093-691X(02)01263-3