Euprosterna elaeasa virus genome sequence and evolution of the Tetraviridae family: emergence of bipartite genomes and conservation of the VPg signal with the dsRNA Birnaviridae family.

摘要: The Tetraviridae is a family of non-enveloped positive-stranded RNA insect viruses that defined by the T=4 symmetry virions. We report complete Euprosterna elaeasa virus (EeV) genome sequence 5698 nt with no poly(A) tail and two overlapping open reading frames, encoding replicase capsid precursor, approximately 67% amino acid identity to Thosea asigna (TaV). N-terminally positioned 17 kDa protein released from precursor NPGP motif. EeV has 40 nm isometric particles composed 58 7 proteins. 3'-end TaV/EeV predicted form conserved pseudoknot. Replicases TaV include newly delineated VPg signal mediating priming synthesis in dsRNA Birnaviridae. Results rooted phylogenetic analysis proteins are presented implicate recombination between monopartite tetraviruses, involving autonomization sgRNA, emergence bipartite tetraviruses. They also used revise taxonomy.