MicroRNA-125a-5p modulates macrophage polarization by targeting E26 transformation-specific variant 6 gene during orthodontic tooth movement.
作者： Wendan He , Nan Zhang , Zhengshen Lin
DOI: 10.1016/J.ARCHORALBIO.2021.105060
关键词:
摘要: Abstract Objective The aim of this study was to investigate the role microRNA-125a-5p (miR-125a-5p) in macrophages during orthodontic tooth movement (OTM). Design Periodontal ligament tissues were collected from patients underwent OTM. cells isolated periodontal tissues. stem normal human impacted third molars. miR-125-5p levels measured by real-time quantitative polymerase chain reaction. impact on macrophage polarization tested alizarin red staining assay. effects and E26 transformation-specific variant 6 gene (ETV6) M1/M2 phenotype markers determined reaction, western blot, flow cytometry analyses. interaction between ETV6 verified using luciferase reporter RNA immunoprecipitation assays. Results miR-125a-5p upregulated under force. Macrophage facilitated osteogenesis cocultured system. Moreover, polarized with M2 conditions. MiR-125a-5p downregulation promoted expression M1 markers, while suppressed markers. Mechanistically, confirmed be a target miR-125a-5p. overexpression increased decreased Furthermore, knockdown reversed inhibitor both macrophages. Conclusions Overall, facilitates bone healing targeting promote polarization.
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