Chitosan as inter-cellular linker to accelerate multicellular spheroid generation in hydrogel scaffold
作者： Yang Liu , Ying Guan , Yongjun Zhang
DOI: 10.1016/J.POLYMER.2015.09.073
关键词:
摘要: Abstract Multicellular spheroids (MCSs) are in vitro tissue models having important biomedical applications. The MCS generation is usually time-consuming because most fabrication methods exploit the inherent ability of cells to self-assemble form spheroids. Here we tried accelerate by chemical pre-aggregation cells. We first developed a method chemically aggregate cells, which involves treatment with NaIO 4 produce surface aldehyde functionalities, and then incubation acrylic acid-modified chitosan (chitosan-AA), new inter-cellular linker. Biocompatibility test indicates chitosan-AA non-toxic even at high concentration. When incubated in presence chitosan-AA, -treated quickly, reaction between amino groups on cell surface. In addition, resulting aggregates remain viability. pre-aggregated were seeded into an situ-formed hydrogel generate MCSs. While it takes several days MCSs control groups, only 1 day for compact group. formed group (231 ± 74 μm Day 4) bigger than those obtained (70 ± 17 μm at 4). grow proliferate faster (P
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sci-hub.se 参考文章(61)
Mark D. Ungrin, Chirag Joshi, Andra Nica, Céline Bauwens, Peter W. Zandstra, Reproducible, Ultra High-Throughput Formation of Multicellular Organization from Single Cell Suspension-Derived Human Embryonic Stem Cell Aggregates PLoS ONE. ,vol. 3, pp. e1565- ,(2008) , 10.1371/JOURNAL.PONE.0001565
C.S. Cho, S.J. Seo, I.K. Park, S.H. Kim, T.H. Kim, T. Hoshiba, I. Harada, T. Akaike, Galactose-carrying polymers as extracellular matrices for liver tissue engineering Biomaterials. ,vol. 27, pp. 576- 585 ,(2006) , 10.1016/J.BIOMATERIALS.2005.06.008
Wen-Yu Lee, Yu-Hsiang Chang, Yi-Chun Yeh, Chun-Hung Chen, Kurt M. Lin, Chieh-Cheng Huang, Yen Chang, Hsing-Wen Sung, The use of injectable spherically symmetric cell aggregates self-assembled in a thermo-responsive hydrogel for enhanced cell transplantation. Biomaterials. ,vol. 30, pp. 5505- 5513 ,(2009) , 10.1016/J.BIOMATERIALS.2009.07.006
Alison Abbott, Biology's new dimension Nature. ,vol. 424, pp. 870- 872 ,(2003) , 10.1038/424870A
J.F.A. Valente, V.M. Gaspar, B.P. Antunes, P. Countinho, I.J. Correia, Microencapsulated chitosan–dextran sulfate nanoparticles for controled delivery of bioactive molecules and cells in bone regeneration Polymer. ,vol. 54, pp. 5- 15 ,(2013) , 10.1016/J.POLYMER.2012.10.032
Ruei-Zhen Lin, Hwan-You Chang, Recent advances in three-dimensional multicellular spheroid culture for biomedical research Biotechnology Journal. ,vol. 3, pp. 1172- 1184 ,(2008) , 10.1002/BIOT.200700228
Xuejun Mo, Qiushi Li, Lena Wai Yi Lui, Baixue Zheng, Chiang Huen Kang, Bramasta Nugraha, Zhilian Yue, Rui Rui Jia, Hong Xia Fu, Deepak Choudhury, Talha Arooz, Jie Yan, Chwee Teck Lim, Shali Shen, Choon Hong Tan, Hanry Yu, Rapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker Biomaterials. ,vol. 31, pp. 7455- 7467 ,(2010) , 10.1016/J.BIOMATERIALS.2010.06.020
Chong Li, Qiuyan Han, Ying Guan, Yongjun Zhang, Thermal gelation of chitosan in an aqueous alkali–urea solution Soft Matter. ,vol. 10, pp. 8245- 8253 ,(2014) , 10.1039/C4SM01336K
Kentaro Hayashi, Yasuhiko Tabata, Preparation of stem cell aggregates with gelatin microspheres to enhance biological functions. Acta Biomaterialia. ,vol. 7, pp. 2797- 2803 ,(2011) , 10.1016/J.ACTBIO.2011.04.013
Vladimir Mironov, Richard P. Visconti, Vladimir Kasyanov, Gabor Forgacs, Christopher J. Drake, Roger R. Markwald, Organ printing: Tissue spheroids as building blocks☆ Biomaterials. ,vol. 30, pp. 2164- 2174 ,(2009) , 10.1016/J.BIOMATERIALS.2008.12.084