SMAD 8 binding to mice Msx1 basal promoter is required for transcriptional activation

摘要: The Msx1 gene in mice has been proven to be induced by BMP (bone morphogenetic protein) proteins, and three binding sites for SMAD, an intracellular signalling transducer, have already identified its promoter. Gel shift analyses were performed they demonstrated that the consensus found very near transcription start site, a region designed BP (basal promoter), is functional nuclear proteins from 10.5, 11.5 13.5 dpc (days post-coitum) embryos. Notably, this occurs only when SMAD-binding sequence maintained, suggesting it required formation of protein complex over BP. Binding purified SMAD 1 SMAD 4 as well supershift assay with SMAD 1/SMAD 5/SMAD 8 antibody proved SMAD present complex. Transfection assays cell cultures fragments driving expression luciferase confirmed presence site activated. A proteomic analysis components after immunoprecipitation several necessary activate including SMAD 8. Our results suggest BMP2/BMP4 through SMAD 8 transcriptional activation mouse gene.