Alarin in cranial autonomic ganglia of human and rat.

摘要: Abstract Extrinsic and intrinsic sources of the autonomic nervous system contribute to choroidal innervation, thus being responsible for control blood flow, aqueous humor production or intraocular pressure. Neuropeptides are involved in this control, amongst those, alarin has been recently introduced. While is present neurons, it not clear if these only source neuronal choroid. Therefore, we here screened presence human cranial ganglia, also rat, a species lacking innervation. Cranial ganglia (i.e., ciliary, CIL; pterygopalatine, PPG; superior cervical, SCG; trigeminal ganglion, TRI) rat were prepared immunohistochemistry against murine alarin, respectively. Additionally, double staining experiments choline acetyltransferase (ChAT), tyrosine hydroxilase (TH), substance P (SP) performed unequivocal identification ganglia. For documentation, confocal laser scanning microscopy was used, while quantitative RT-PCR applied confirm immunohistochemical data detect mRNA expression. In humans, alarin-like immunoreactivity (alarin-LI) detected neurons nerve fibers stroma, but CIL, PPG, SCG TRI. alarin-LI minority (CIL: 3.5%; PPG: 0.4%; SCG: 1.9%; TRI: 1%). qRT-PCR confirmed low expression level Since absent few consider impact extrinsic ocular innervation those species. Nevertheless, seems important where could serve as marker.