Colorimetric and visual mercury(II) assay based on target-induced cyclic enzymatic amplification, thymine-Hg(II)-thymine interaction, and aggregation of gold nanoparticles.
作者： Xiaolei Song , Yu Wang , Su Liu , Xue Zhang , Haiwang Wang
DOI: 10.1007/S00604-018-3193-8
关键词:
摘要: A colorimetric biosensor and visual test is described for the determination of mercury(II). It relies on specific thymine-Hg(II)-thymine (T-Hg2+-T) interaction which induces a cyclic amplification process (caused by enzyme exonuclease III) aggregation gold nanoparticles. These results in color change from red to violet. Under optimized conditions, this assay (best performed at 524 nm) has detection limit as low 0.9 nM with range over 4 orders magnitude (from 1 10 μM).
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