An in vitro assay to measure the viability of KHT tumor cells not previously exposed to culture conditions

摘要: An in vitro colony assay which measures the viability of KHT fibrosarcoma cells is described. Single cell suspensions are prepared from tumors growing C3H mice with no previous adaptation for growth. The involves incubating at 37° 2 wk Petri dishes containing a semisolid matrix α medium, fetal calf serum, and Bacto agar. Colonies 20 or more then counted under microscope. plating efficiency ranges 5-40% based on number viable plated as determined by dye exclusion. However, this dye-exclusion technique may overestimate since separated velocity sedimentation 50-90%. Survival curves have been irradiated air nitrogen. ${\rm D}_{0}\text{'}{\rm s}$ 167 478 rads extrapolation numbers 1.6 1.4 were obtained aerobic anoxic curves, respectively. These reasonable agree...