Reassessing Germinal Centre Reaction Concepts

摘要: To determine the number of B cells seeding germinal centres, different authors have used immunohistology centre sections in conjunction with assuming a binomial distribution fractions two phenotypically distinct cell populations participating given immune response. This approach further assumed that centres are closed to continuous entry. Using such model, it has been concluded contain eight clones, figure is usually taken and cited as being essentially correct. The present re-evaluation those related experiments lead an extended mathematical model. model includes estimation errors created by data sampling, new parameters take into account possible mistakes classification single population GC sections, likely variability cells. Fitting this experimental resulted estimated mean ⟨n⟩ = 23–37 seeder