Protocol of triticale (x Triticosecale Wittmack) microspore culture

摘要: Triticale (x Triticosecale Wittmack) is a spontaneous synthetic amphiploid cereal that has been considerably improved through breeding. currently grown on about 2 million hectares worldwide. After the initial period, intensive breeding research triticale was started in early 1950s by Arpad Kiss Hungary. Nowadays, centre of European Poland. Polish breeders released excellent varieties have had good performance, especially Europe. Since induction first anther culture derived haploid plantlets Wang et al. (1973), method essentially modified and improved. Theoretically, two vitro cell tissue methods are used to induce androgenesis: microspore culture. The seems be more laborious. blender isolation protocol provides sufficient number microspores avoid necessity for isolation. In cereals, microblending earlier established barley (Olsen, 1991) wheat (Ziauddin al.,1992), confirming its potential. present describes an process blender-isolated leading doubled lines from embryo-like structures (ELS). Embryogenesis observed isolated microspores. This phenomenon also occurs other cereals. From ELS green plants can regenerated, but albinism still typical problem Depending genotype, than half regenerants may albino.