Inflammatory leukocytic recruitment and diffuse neuronal degeneration are separate pathological processes resulting from traumatic brain injury

摘要: The present study characterized whether inflammatory leukocytic infiltration is temporally and regionally correlated with neuronal degeneration and/or blood brain barrier (BBB) breakdown resulting from traumatic injury. Adult rats were sacrificed at 5 min, 2, 4, 12, 24, 72 hr after lateral fluid percussion BBB breakdown, leukocyte assessed using immunocytochemistry, silver impregnation toluidine blue eosin staining. occurred concomitantly in injured cortex, hippocampus, along the dorsolateral quadrant of diencephalon. However, within deep diencephalic structures transpired absence IgG extravasation. Neutrophils observed only regions exhibiting damage first apparent cortex hippocampus between 2-12 posttrauma lining vasculature filling subarachnoid/subdural spaces. then migrated damaged into traumatized cortical hippocampal parenchyma by 24 Macrophages also completely filled lesion site not as abundant throughout found focal hemorrhage hr. Finally, neutrophils did migrate to that showed no despite extensive degeneration. Indeed, elicits recruitment experiencing concomitant In summary, diffuse are separate pathological processes