Electrochemical assay of plasmin activity and its kinetic analysis.
作者： Keiichi Ohtsuka , Iwao Maekawa , Michinori Waki , Shigeori Takenaka
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摘要: A sensitive and convenient electrochemical assay of plasmin activity its kinetic analysis are described. Thus, a ferrocenyl peptide substrate (FcPS) having plasmin-specific sequence, Lys-Thr-Phe-Lys, Cys residue was prepared immobilized on gold electrode through the sulfur-gold linkage. The obtained showed redox signal based ferrocene moiety, suggesting immobilization FcPS electrode. After treatment this with plasmin, decreased in proportion to an increase amount plasmin. detection limit for system as low 50 ng/ml or 0.15 mU/ml. Real-time monitoring reaction could also be achieved, parameters enzymatic determined; example, k(cat)/K(m) value 0.063 microM(-1) s(-1). Furthermore, quantitative streptokinase plasminogen activator demonstrated by using system.
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