Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium.
作者： Changrim Lee , Maria C. Edman , Gordon W. Laurie , Sarah F. Hamm-Alvarez , J. Andrew MacKay
DOI: 10.3390/IJMS21176157
关键词:
摘要: Lacripep is a therapeutic peptide derived from the human tear protein, Lacritin. interacts with syndecan-1 and induces mitogenesis upon removal of heparan sulfates (HS) that are attached at extracellular domain syndecan-1. The presence HS prerequisite for clustering stimulates exosome biogenesis release. Therefore, syndecan-1-mediated versus HS-mediated assumed to be mutually exclusive. This study introduces biosynthesized fusion between an elastin-like polypeptide named LP-A96, evaluates its activity on cell motility enhancement biogenesis. LP-A96 activates both downstream pathways in dose-dependent manner. HCE-T cells high confluence treated 1 μM enhanced equipotent Lacripep. However, low density generated 210-fold higher number exosomes compared those As monovalent capable enhancing but not biogenesis, activation by only suggests utility as novel molecular tool Lacritin biology corneal epithelium also implies potential can further improve ocular surface health through induction exosomes.
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sci-hub.st 参考文章(44)
M. Baalousha, F.V.D. Kammer, M. Motelica-Heino, H.S. Hilal, P. Le Coustumer, Size fractionation and characterization of natural colloids by flow-field flow fractionation coupled to multi-angle laser light scattering Journal of Chromatography A. ,vol. 1104, pp. 272- 281 ,(2006) , 10.1016/J.CHROMA.2005.11.095
Atsuko Fujii, Ayumi Morimoto-Tochigi, Ryan D. Walkup, Thomas R. Shearer, Mitsuyoshi Azuma, Lacritin-Induced Secretion of Tear Proteins From Cultured Monkey Lacrimal Acinar Cells Investigative Ophthalmology & Visual Science. ,vol. 54, pp. 2533- 2540 ,(2013) , 10.1167/IOVS.12-10394
Dipannita Dutta, Julie G. Donaldson, Search for inhibitors of endocytosis: Intended specificity and unintended consequences Cellular logistics. ,vol. 2, pp. 203- 208 ,(2012) , 10.4161/CL.23967
Ariel Savina, Marcelo Furlán, Michel Vidal, Maria I. Colombo, Exosome release is regulated by a calcium-dependent mechanism in K562 cells. Journal of Biological Chemistry. ,vol. 278, pp. 20083- 20090 ,(2003) , 10.1074/JBC.M301642200
A CHILKOTI, T CHRISTENSEN, J MACKAY, Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology. Current Opinion in Chemical Biology. ,vol. 10, pp. 652- 657 ,(2006) , 10.1016/J.CBPA.2006.10.010
Keyang Chen, Kevin Jon Williams, Molecular Mediators for Raft-dependent Endocytosis of Syndecan-1, a Highly Conserved, Multifunctional Receptor Journal of Biological Chemistry. ,vol. 288, pp. 13988- 13999 ,(2013) , 10.1074/JBC.M112.444737
Ana Guzman-Aranguez, Basilio Colligris, Jesús Pintor, Contact Lenses: Promising Devices for Ocular Drug Delivery Journal of Ocular Pharmacology and Therapeutics. ,vol. 29, pp. 189- 199 ,(2013) , 10.1089/JOP.2012.0212
Gordon W. Laurie, Leslie A. Olsakovsky, Brian P. Conway, Robert L. McKown, Kazuko Kitagawa, Jason J. Nichols, Dry Eye and Designer Ophthalmics Optometry and Vision Science. ,vol. 85, pp. 643- 652 ,(2008) , 10.1097/OPX.0B013E318181AE73
Francisco Velez V, Jeffrey A. Romano, Robert L. McKown, Kari Green, Liwen Zhang, Ronald W. Raab, Denise S. Ryan, Cindy M. L. Hutnik, Henry F. Frierson, Gordon W. Laurie, Tissue transglutaminase is a negative regulator of monomeric lacritin bioactivity. Investigative Ophthalmology & Visual Science. ,vol. 54, pp. 2123- 2132 ,(2013) , 10.1167/IOVS.12-11488
Liat Fux, Neta Ilan, Ralph D. Sanderson, Israel Vlodavsky, Heparanase: busy at the cell surface Trends in Biochemical Sciences. ,vol. 34, pp. 511- 519 ,(2009) , 10.1016/J.TIBS.2009.06.005