Distinct utilization of effectors and biological outcomes resulting from site-specific Ras activation: Ras functions in lipid rafts and Golgi complex are dispensable for proliferation and transformation.

摘要: Ras proteins are distributed in different types of plasma membrane microdomains and endomembranes. However, how microlocalization affects the signals generated by its subsequent biological outputs is largely unknown. We have approached this question selectively targeting RasV12 to cellular sublocalizations. show here that compartmentalization dictates utilization effectors intensity signals. Activated can evoke enhanced proliferation transformation from most platforms, with exception Golgi complex. Furthermore, promote survival emanate primarily endoplasmic reticulum pool. In addition, we investigated need for pools endogenous conveyance upstream mitogenic transforming Using targeted RasN17 inhibitory mutants physiological contexts such as H-Ras/N-Ras double knockout fibroblasts, demonstrate functions at lipid rafts complex fully dispensable transformation.