PEP-1-SIRT2-induced matrix metalloproteinase-1 and -13 modulates type II collagen expression via ERK signaling in rabbit articular chondrocytes

摘要: Abstract Matrix metalloproteinases (MMPs) are critical for the degradation of extracellular matrix (ECM), which includes cartilage-specific collagen types I, II and XI. We previously found that PEP-1-sirtuin (SIRT)2 could induce dedifferentiation articular chondrocytes; however, underlying mechanisms remains unclear. addressed this in present study by examining association between PEP-1-SIRT2 expression MMP-1 MMP-13 type rabbit chondrocytes. increased -13 a dose- time-dependent manner, as determined western blotting. A similar trend levels was observed cultures during expansion to four passages. Pharmacological inhibition blocked PEP-1–SIRT2-induced decrease level. Phosphorylation regulated kinase (ERK) PEP-1–SIRT2; treatment with mitogen-activated protein inhibitor PD98059 suppressed while restoring passage 2 cells. These results suggest PEP-1–SIRT2 promotes MMP-induced via ERK signaling