The Membrane-Bound Ectopeptidase CPM as a Marker Of Macrophage Maturation in vitro And in vivo

摘要: During terminal maturation of human blood monocytes into macrophages, a multitude phenotypic and functional changes occurs: cells increase in size, they enhance their capacity for phagocytosis tumor cytotoxicity but decrease ability T-lymphocytestimulation. The pattern secreted cytokines is shifted as the profile surface antigens. We recently identified carboxypeptidase M (CPM) macrophage maturation-associated antigendetected by mAb MAX.1/MAX.11. CPM, phosphoinositol-linked ectopeptidase, able to process different substrates, among them immunologically important peptides like bradykinin, anaphylatoxins enkephalins. It was previously shown be expressed placenta, lung, kidney. CPM detected MAX.1/11 shows strong expression on monocyte-derived macrophages vitro vivo accompanying T-lymphocyte activation during allogeneic transplant rejection or allergic alveolitis. hi contrast, its suppressed some types cells. seems correlate with cytotoxic functions. However, biological importance difficult predict. A wide range biologically active are cleaved relevance peptide processing an immune reaction only poorly understood. generation analysis CPM-deficient animals might improve our understanding function. Therefore we cloned cDNA murine homologue CPM. mCPM undetectable primary cell-lines, suggesting that function not conserved between mouse macrophages.