Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines.
作者： Ming-jiang Xu , Kohichiro Tsuji , Takahiro Ueda , Yoh-suke Mukouyama , Takahiko Hara
DOI: 10.1182/BLOOD.V92.6.2032.418K29_2032_2040
关键词:
摘要: We report here on a novel stromal cell line, AGM-S3, derived from the aorta-gonad-mesonephros (AGM) region of 10.5 days postcoitum (dpc) mouse embryo. The AGM-S3 cells promoted production hematopoietic progenitors and day-12 spleen colony-forming Lin-c-Kit+Sca-1(+) murine primitive cells. They also supported for 6 weeks generation human multipotential cord blood CD34(+)CD38(-) Human long-term repopulating stem (LTR-HSC) with potential to reconstitute hematopoiesis in NOD/SCID mice were maintained at least 4 weeks. Flow cytometric analysis showed that CD13, vascular cellular adhesion molecule-1, Sca-1 expressed Because factor, interleukin-6 (IL-6), oncostatin M, but not IL-3, IL-11, leukemia- inhibitory granulocyte colony-stimulating granulocyte-macrophage thrombopoietin, Flk2 ligand detected reverse transcription-polymerase chain reaction cells, seem express species-cross reactive molecule(s) other than cytokines examined which act progenitor/stem This line is expected elucidate molecular mechanisms regulating early pave way developing strategies expansion transplantable HSC.
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