Responses of growth inhibition and antioxidant gene expression in earthworms (Eisenia fetida) exposed to tetrabromobisphenol A, hexabromocyclododecane and decabromodiphenyl ether.
作者： Ya-juan Shi , Xiang-bo Xu , Xiao-qi Zheng , Yong-long Lu
DOI: 10.1016/J.CBPC.2015.06.005
关键词:
摘要: Tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD) and decabromodiphenyl ether (BDE 209), suspected ubiquitous contaminants, account for the largest volume of brominated flame retardants (BFRs) since penta-BDE octa-BDE have been phased out globally. In this paper, growth inhibition gene transcript levels antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT)) stress-response involved in prevention oxidative stress (Hsp70) earthworms (Eisenia fetida) exposed to TBBPA, HBCD BDE 209 were measured identify toxicity effects selected BFRs on earthworms. The treated by TBBPA at 200 400 mg/kg dw inhibited rate 13.7% 22.0% respectively, while there was no significant 209. (P < 0.01) up-regulation SOD expression level observed 50 (1.77-fold) (2.06-fold). Hsp70 significantly up-regulated when concentration 50-200 (2.16-2.19-fold) (2.61-fold). No variation CAT all treatments observed, neither does target Assessed changes mRNA encoding genes earthworms, showed greatest toxicity, followed 209, consistent with trends molecular properties. results help understand mechanism defense. (C) 2015 Elsevier Inc. All rights reserved.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(41)
J. Homa, S.R. Stürzenbaum, A.J. Morgan, B. Plytycz, Disrupted homeostasis in coelomocytes of Eisenia fetida and Allolobophora chlorotica exposed dermally to heavy metals European Journal of Soil Biology. ,vol. 43, ,(2007) , 10.1016/J.EJSOBI.2007.08.027
F. Brulle, G. Mitta, C. Cocquerelle, D. Vieau, S. Lemière, A. Leprêtre, F. Vandenbulcke, Cloning and Real-Time PCR Testing of 14 Potential Biomarkers inEisenia fetidaFollowing Cadmium Exposure Environmental Science & Technology. ,vol. 40, pp. 2844- 2850 ,(2006) , 10.1021/ES052299X
P. Gonzalez, Y. Dominique, J. C. Massabuau, A. Boudou, J. P. Bourdineaud, Comparative Effects of Dietary Methylmercury on Gene Expression in Liver, Skeletal Muscle, and Brain of the Zebrafish (Danio rerio) Environmental Science & Technology. ,vol. 39, pp. 3972- 3980 ,(2005) , 10.1021/ES0483490
Christopher L Rowe, William A Hopkins, Caralyn Zehnder, Justin D Congdon, Metabolic costs incurred by crayfish (Procambarus acutus) in a trace element-polluted habitat: further evidence of similar responses among diverse taxonomic groups. Comparative Biochemistry and Physiology C-toxicology & Pharmacology. ,vol. 129, pp. 275- 283 ,(2001) , 10.1016/S1532-0456(01)00204-6
Wenguang Xiong, Xueyao Ding, Yiming Zhang, Yongxue Sun, Ecotoxicological effects of a veterinary food additive, copper sulphate, on antioxidant enzymes and mRNA expression in earthworms Environmental Toxicology and Pharmacology. ,vol. 37, pp. 134- 140 ,(2014) , 10.1016/J.ETAP.2013.11.014
Jae-Sung Rhee, Sheikh Raisuddin, Kyun-Woo Lee, Jung Soo Seo, Jang-Seu Ki, Il-Chan Kim, Heum Gi Park, Jae-Seong Lee, Heat shock protein (Hsp) gene responses of the intertidal copepod Tigriopus japonicus to environmental toxicants. Comparative Biochemistry and Physiology C-toxicology & Pharmacology. ,vol. 149, pp. 104- 112 ,(2009) , 10.1016/J.CBPC.2008.07.009
Xianchuan Xie, Yingxin Wu, Mengying Zhu, You-kuan Zhang, Xiaorong Wang, Hydroxyl radical generation and oxidative stress in earthworms (Eisenia fetida) exposed to decabromodiphenyl ether (BDE-209). Ecotoxicology. ,vol. 20, pp. 993- 999 ,(2011) , 10.1007/S10646-011-0645-X
Sebastián E. Sabatini, Ángela B. Juárez, Maria R. Eppis, Laura Bianchi, Carlos M. Luquet, María del Carmen Ríos de Molina, Oxidative stress and antioxidant defenses in two green microalgae exposed to copper. Ecotoxicology and Environmental Safety. ,vol. 72, pp. 1200- 1206 ,(2009) , 10.1016/J.ECOENV.2009.01.003
Cynthia A. de Wit, Mehran Alaee, Derek C.G. Muir, Levels and trends of brominated flame retardants in the Arctic Chemosphere. ,vol. 64, pp. 209- 233 ,(2006) , 10.1016/J.CHEMOSPHERE.2005.12.029
Robin J Law, Adrian Covaci, Stuart Harrad, Dorte Herzke, Mohamed A-E Abdallah, Kim Fernie, Leisa-Maree L Toms, Hidetaka Takigami, None, Levels and trends of PBDEs and HBCDs in the global environment: Status at the end of 2012 Environment International. ,vol. 65, pp. 147- 158 ,(2014) , 10.1016/J.ENVINT.2014.01.006