Characterization of two kcnk3 genes in Nile tilapia (Oreochromis niloticus): Molecular cloning, tissue distribution, and transcriptional changes in various salinity of seawater.

摘要: Abstract As one important member of the two-pore-domain potassium channel (K2P) family, subfamily K 3 (KCNK3) has been reported for thermogenesis regulation, energy homeostasis, membrane potential conduction, and pulmonary hypertension in mammals. However, its roles fishes are far less examined published. In present study, we identified two kcnk3 genes (kcnk3a kcnk3b) an euryhaline fish, Nile tilapia (Oreochromis niloticus), by molecular cloning, genomic survey laboratory experiments to investigate their osmoregulation. We obtained full-length coding sequences kcnk3a kcnk3b (1209 1173 bp), which encode 402 390 amino acids, respectively. Subsequent multiple sequence alignments, putative 3D-structure model prediction, phylogenetic analysis confirmed that paralogs widely presented fish genomes. Interestingly, a DNA fragment inversion cluster was found Cypriniforme comparison with other fishes. Quantitative real-time PCRs demonstrated both were detected all tissues similar distribution pattern, highest transcriptions observed heart. Meanwhile, gill proved have transcriptional change pattern response various salinity seawater, implying they might be involved Furthermore, three predicted transcription factors (arid3a, arid3b, arid5a) also showed as target salinity, suggesting positive regulatory roles. summary, first time characterized tilapia, involvement osmoregulation this economically fish.