Tissue culture of human neurocytomas induces the expression of glial fibrilary acidic protein.

摘要: Summary. Cell cultures were established from three human neurocytoma specimens (primary and recurrent). The phenotypic evolution was analyzed by immunocytology in different culture conditions the presence absence of serum including addition epidermal growth factor, rat caudate extract, retinoic acid, N-acetyl cystein. cells grown on glass cover slides or an extracellular matrix (ECM) bovine corneal endothelial cells. Immunostainings performed after overnight incubation repeated 5 10 days culture. compared to oligoastrocytoma also arising at foramen Monro ependymoma frontal lateral ventricle, two tumors supposedly originating same tissue as neurocytoma. After incubation, 90% positive for A2B5 synaptophysin. GFAP reactivity appeared periphery cell processes less than 1% staining patterns morphology nearly identical under conditions. days, almost all strongly GFAP, while number remaining synaptophysin unchanged earlier time point. Again, there no fundamental differences between At this point, maintained ECM their counterparts untreated with results, although many fewer had attached. An immuno-reactive pattern found day 10. In contrast cultures, immediate strong signal both mixed glioma ependymoma. positive, but absent. Because negative immunohistochemistry, it is concluded that neurocytomas may represent a neuronoglial precursor tumor switches its phenotype astroglial differentiation despite very diverse