Isolation and structural characterization of calcitonin gene-related peptide from the brain and intestine of the frog, Rana ridibunda.
作者： J. Michael Conlon , Marie-Christine Tonon , Hubert Vaudry
DOI: 10.1016/0196-9781(93)90148-A
关键词:
摘要: Calcitonin gene-related peptide (CGRP) was isolated in a single molecular form from an extract of the whole brain and small intestine European green frog, Rana ridibunda. The primary structure established as: Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His10-Arg-Leu-Ala-Asp-Phe-Leu- Ser-Arg-Ser-Gly20-Gly-Met-Ala-Lys-Asn-Asn-Phe-Val-Pro-Thr30- Asn-Val-Gly-Ser-Ala-Phe-NH2. Frog CGRP shows only two amino acid substitutions (Val22-->Met Gly23-->Ala) compared with chicken CGRP, as deduced nucleotide sequence cloned cDNA. Asp14 residue, which leads to enhanced biological potency is also present frog peptide. data show that has been strongly conserved during evolution vertebrates.
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