Activation of Olfactory Receptors on Mouse Pulmonary Macrophages Promotes Monocyte Chemotactic Protein-1 Production

摘要: Background Emerging evidence suggests that non-olfactory tissues and cells can express olfactory receptors (ORs), however, the exact function of ectopic OR expression remains unknown. We have previously shown in mouse models a unique cooperation between interferon-γ (IFN-γ) lipopolysaccharide (LPS) drives activation pulmonary macrophages leads to induction pathogenic responses respiratory tract. Further, through gene array studies, we by these molecules results selective number ORs. In this study, validated ORs airway response IFN-γ LPS (γ/LPS) stimulation, further explored effect odorant stimulation on macrophage function. Methodology/Principal Findings OR IFN-γ, or γ/LPS treatments was assessed microarray q-PCR. (e.g. OR622) confirmed visualization immunofluoresence assays. Functional odorants were quantifying inflammatory cytokine chemokine using q-PCR cell migration modified Boyden chamber assay. Our demonstrate eight are expressed at basal levels both macrophages, cooperatively increased expression. Pulmonary exposed combined treatment γ/LPS+octanal (an odorant) exhibited 3-fold increase MCP-1 protein production, compared treated with alone. Supernatants from also vitro. Conclusions/Significance Eight different is upregulated synergistic action γ/LPS. Octanal production motility macrophages. suggest may mediate regulating migration.