In-situ transmission electron microscopy of liposomes in an aqueous environment.
作者： Sarah M. Hoppe , Darryl Y. Sasaki , Aubrianna N. Kinghorn , Khalid Hattar
DOI: 10.1021/LA401288G
关键词:
摘要: The characterization of liposomes was undertaken using in-situ microfluidic transmission electron microscopy. Liposomes were imaged without contrast enhancement staining or cryogenic treatment, allowing for the observation functional in an aqueous environment. stability and quality liposome structures observed found to be highly dependent on surface chemistries within liquid cell. successful imaging suggests potential extension TEM a wide variety other biological soft matter systems processes.
acs.org
osti.gov
sci-hub.se 参考文章(25)
K.L. KLEIN, I.M. ANDERSON, N. DE JONGE, Transmission electron microscopy with a liquid flow cell Journal of Microscopy. ,vol. 242, pp. 117- 123 ,(2011) , 10.1111/J.1365-2818.2010.03484.X
Russell L. Steere, Electron microscopy of structural detail in frozen biological specimens. Journal of Cell Biology. ,vol. 3, pp. 45- 60 ,(1957) , 10.1083/JCB.3.1.45
Giovanni Tosi, Massimo Tonelli, Lucia Bondioli, Adele Mucci, Flavio Forni, Maria Angela Vandelli, Barbara Ruozi, AFM phase imaging of soft-hydrated samples: A versatile tool to complete the chemical-physical study of liposomes Journal of Liposome Research. ,vol. 19, pp. 59- 67 ,(2009) , 10.1080/08982100802584071
D. R. Shnek, D. W. Pack, D. Y. Sasaki, F. H. Arnold, Specific Protein Attachment to Artificial Membranes via Coordination to Lipid-Bound Copper(II) Langmuir. ,vol. 10, pp. 2382- 2388 ,(1994) , 10.1021/LA00019A058
Andreas Bremer, Christian Henn, Andreas Engel, Wolfgang Baumeister, Ueli Aebi, Has negative staining still a place in biomacromolecular electron microscopy Ultramicroscopy. ,vol. 46, pp. 85- 111 ,(1992) , 10.1016/0304-3991(92)90008-8
Carl C. Hayden, Jane S. Hwang, Elisa A. Abate, Michael S. Kent, Darryl Y. Sasaki, Directed formation of lipid membrane microdomains as high affinity sites for His-tagged proteins. Journal of the American Chemical Society. ,vol. 131, pp. 8728- 8729 ,(2009) , 10.1021/JA901157C
Roberta A. Parente, Mathias Höchli, Barry R. Lentz, Morphology and phase behavior of two types of unilamellar vesicles prepared from synthetic phosphatidylcholines studied by freeze-fracture electron microscopy and calorimetry Biochimica et Biophysica Acta. ,vol. 812, pp. 493- 502 ,(1985) , 10.1016/0005-2736(85)90324-4
Yeshayahu Talmon, Staining and drying-induced artifacts in electron microscopy of surfactant dispersions Journal of Colloid and Interface Science. ,vol. 93, pp. 366- 382 ,(1983) , 10.1016/0021-9797(83)90420-4
Dan Peer, Jeffrey M. Karp, Seungpyo Hong, Omid C. Farokhzad, Rimona Margalit, Robert Langer, Nanocarriers as an emerging platform for cancer therapy Nature Nanotechnology. ,vol. 2, pp. 751- 760 ,(2007) , 10.1038/NNANO.2007.387
L. Belkoura, C. Stubenrauch, R. Strey, Freeze fracture direct imaging: a new freeze fracture method for specimen preparation in cryo-transmission electron microscopy. Langmuir. ,vol. 20, pp. 4391- 4399 ,(2004) , 10.1021/LA0303411