Rapid detection of Salmonella using a redox cycling-based electrochemical method

摘要: Abstract An electrochemical method based on redox cycling combined with immunomagnetic separation and pre-concentration was developed for rapid sensitive detection of Salmonella. Electrochemical methods the bacteria offer advantages instant quantification minimal equipment. Unfortunately, limits are often poor compare to other transduction such as fluorescence chemiliuminescence. We demonstrated an which is both has a low limit detection. A two-step strategy, included immunomagentic used amplify signal. Magnetic beads modified anti-Salmonella antibodies were Salmonella from phosphate buffered saline (PBS) agricultural water. Then conjugated alkaline phosphatase employed labeling had been captured by magnetic beads. Alkaline (ALP) catalyzed substrate l -ascorbic acid 2-phosphate (AAP) electroactive species (AA) while tris(2-carboxyethyl)phosphine (TCEP) facilitated regeneration AA gold electrode form resulting in amplified Under optimal conditions, PBS buffer well water detected. The this approach approximately 7.6 × 102 CFU/mL 6.0 × 102 CFU/mL water, respectively, without pre-enrichment 3 h. When pre-enriched 4 h, 10 CFU/mL.