TGF-beta 1 promotes in vitro generation of dendritic cells by protecting progenitor cells from apoptosis.

摘要: Our previous studies demonstrated that TGF-beta 1 is required for efficient in vitro generation of dendritic cells (DC) from CD34+ progenitor under serum-free conditions. Here we show promotes the growth and differentiation DC primarily by protecting viability precursors not enhancing their proliferative response. Addition to TNF-alpha, granulocyte-macrophage CSF, stem cell factor-supplemented cultures had no significant effect on proportions cycling cells. Already at 72 h culture, however, proportion apoptotic was reduced more than 60% presence 1. This early protective correlates with outgrowth higher numbers CD1a+ day 7 culture. It also a significantly Fas/APO-1 expression cultured In contrast, granulomonocytic cells, arising these culture conditions, are affected such an extent. They found equal proportions, both absence The striking growth-promoting could only be observed when TNF-alpha were present cultures. 1, rather inhibited enhanced expansion. Thus, optimal development occur, all four cytokines must obviously act concert combination seems particularly critical.