Epitope mapping of Grapevine virus A capsid protein
作者： M. Dell'Orco , P. Saldarelli , A. Minafra , D. Boscia , D. Gallitelli
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摘要: Previously characterized monoclonal antibodies (MAbs) to Grapevine virus A (GVA) showed a differential reactivity against intact or partially destabilized particles [2]. In the present study, this was confirmed and several peptides reacting with panel of four different were identified by PEPSCAN method epitope mapping. Oligopeptide sequences comprised between coat protein residues 61 (V) 72 (T) recognized all tested. One these (VGPKASK) also reactive when expressed on recombinant phage as fusion pVIII. The specificity sequence for antibody binding demonstrated competitive-ELISA using one GVA MAbs. results study suggest that carry highly structured centered common peptide region sequence.
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