Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of Central India

摘要: Soybean cultivation in many zones of India shows occurrence native rhizobia besides other exotically adapted strains. In the current study, 22 rhizobial isolates (recovered from 12 different soybean growing sites) and 8 refer ence strains were selected for biochemical metabolic characterization. Of isolates, 18 recovered as fast while rest slow based on bromothymol blue (BTB) test. Unlike earlier belief that have no ability to grow glucose peptone agar medium, this some reference grew well medium. Similarly, when all subjected ketolactose test, found show growth contrast, C-utilization pattern (15 carbohydrates) a remarkable diversity was observed among study. The clustering matching analysis showed most with strains, few be unique hence not any Such suggests metabolically distinct types commonly known (B. japonicum, B. elkanii S. fredii) further validation is suggested through 16SrRNA gene sequencing technique.