In vitro human cell responses to a low-dose photodynamic treatment vs. mild H2O2 exposure

摘要: Abstract Photodynamic treatments allow control of the amount reactive oxygen species (ROS) produced through photosensitizer concentration and light dose delivered to target. In this way low ROS doses can be achieved in situ study cell responses related redox regulation. a comparison has been made between different low-dose photodynamic treatment both relatively high concentrations H2O2 human immortalized keratinocytes (HaCaT). The obtained results show that induces stimulating response roughly equivalent by exposing cells 10−5 M H2O2. Higher gave rise concentration-dependent deleterious effects on cultures. Of importance is did not produce genotoxic damage, as measured micronuclei frequency, while cultures exposed displayed significant increase with micronuclei. summary, promotes proliferation but does incur excessive clastogenic lesions observed after exposure. It therefore proposed promising alternative direct exposure signalling.