miR-30 Family microRNAs Regulate Myogenic Differentiation and Provide Negative Feedback on the microRNA Pathway

摘要: microRNAs (miRNAs) are short non-coding RNAs that can mediate changes in gene expression and required for the formation of skeletal muscle (myogenesis). With goal identifying novel miRNA biomarkers disease, we profiled using miRNA-seq gastrocnemius muscles dystrophic mdx4cv mice. After a down-regulation miR-30 family (miR-30a-5p, -30b, -30c, -30d -30e) when compared to C57Bl/6 (WT) mice, found overexpression miRNAs promotes differentiation, while inhibition restricts differentiation myoblasts vitro. Additionally, coordinately down-regulated during vivo models injury (barium chloride injection) disuse atrophy (hindlimb suspension). Using bioinformatics tools vitro studies, identified validated Smarcd2, Snai2 Tnrc6a as targets. Interestingly, show by targeting Tnrc6a, negatively regulate pathway modulate both activity muscle-specific miR-206 levels protein synthesis. These findings indicate may be an interesting biomarker perturbed homeostasis disease.