Expression of hepatitis B virus surface antigen in adult rat liver. Co-introduction of DNA and nuclear protein by a simplified liposome method.

摘要: We established a simple and efficient method for gene transfer in vitro (to cultured cells) vivo an adult organ) using liposomes. Plasmid DNA proteins were efficiently co-encapsulated liposomes by agitation sonication, co-introduced into cells hemagglutinating virus of Japan (HVJ)-mediated membrane fusion. Introduction the Escherichia coli beta-galactosidase with non-histone chromosomal protein high mobility group 1 (HMG1) LLCMK2 resulted about 3 times higher activity than that on introduction alone. Two days after injection HVJ-liposomes containing HMG1 under perisplanchnic rat liver, hepatic near site found 5-bromo-4-chloro-3-indolyl beta-D-galactoside staining to have activity. After similar hepatitis B surface antigen (HBsAg) HMG1, HBsAg was detected serum 9 maximum 25-45 ng/ml day 2 injection.