Quantitative Detection of Class-Specific Rheumatoid Factors and IgA Rheumatoid Factor Subclasses

摘要: Summary An Enzyme-Linked-Immuno-Sorbent-Assay (ELISA) for the quantitative detection of rheumatoid factors (RF) and an ELISA measurement IgA-RF subclasses was developed. In sera from 108 patients with arthritis (RA) whom 47 had a positive latex agglutination assay, 100 healthy controls 119 diseased (49 systemic lupus erythematosis (SLE), 20 osteoarthritis 50 bronchial asthma) levels IgG-, IgA-, IgM-, IgA1- IgA2-RF were evaluated by ELISA. serum samples RA test, elevated IgM-RF could be demonstrated in 94%, 91% 98% respectively. negative 72%, 69% 8% latter patient group increased one or more RF-isotypes detected 82% patients. less than three investigated except that SLE 25% 64% IgG-and Highly significant correlations found between as those Rose-Waaler assays. Positive also investigated. 94% 62% 36% 52% SLE, 41% 37% A correlation but not