Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases.
作者： Larry A. Feig
DOI: 10.1038/10018
关键词:
摘要: One of the most powerful ways studying function a protein is to specifically block its activity within cells. Over past decade, dominant-inhibitory proteins have emerged as popular tools with which accomplish this task; these mutated interfere their normal cellular counterparts or that interact them. This approach has been used extensively in elucidation signal-transduction cascades, such those involving Ras-family proteins. Here I discuss power and potential pitfalls using Ras
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Yi Zheng, Matthew J. Hart, Richard A. Cerione, Guanine nucleotide exchange catalyzed by dbl oncogene product Methods in Enzymology. ,vol. 256, pp. 77- 84 ,(1995) , 10.1016/0076-6879(95)56011-4
Studies on the structure and mechanism of H-ras p21. Philosophical Transactions of the Royal Society B. ,vol. 336, pp. 3- 11 ,(1992) , 10.1098/RSTB.1992.0037
C L Farnsworth, L A Feig, Dominant inhibitory mutations in the Mg(2+)-binding site of RasH prevent its activation by GTP. Molecular and Cellular Biology. ,vol. 11, pp. 4822- 4829 ,(1991) , 10.1128/MCB.11.10.4822
S Powers, K O'Neill, M Wigler, Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 9, pp. 390- 395 ,(1989) , 10.1128/MCB.9.2.390
C C Lai, M Boguski, D Broek, S Powers, Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins. Molecular and Cellular Biology. ,vol. 13, pp. 1345- 1352 ,(1993) , 10.1128/MCB.13.3.1345
J Szeberényi, H Cai, G M Cooper, Effect of a dominant inhibitory Ha-ras mutation on neuronal differentiation of PC12 cells. Molecular and Cellular Biology. ,vol. 10, pp. 5324- 5332 ,(1990) , 10.1128/MCB.10.10.5324
J.P. Noel, H.E. Hamm, P.B. Sigler, The 2.2 A crystal structure of transducin-alpha complexed with GTP gamma S. Nature. ,vol. 366, pp. 654- 663 ,(1994) , 10.2210/PDB1TND/PDB
L A Quilliam, K Kato, K M Rabun, M M Hisaka, S Y Huff, S Campbell-Burk, C J Der, Identification of residues critical for Ras(17N) growth-inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors Molecular and Cellular Biology. ,vol. 14, pp. 1113- 1121 ,(1994) , 10.1128/MCB.14.2.1113
Nina van den Berghe, Robbert H Cool, Gudrun Horn, Alfred Wittinghofer, Biochemical characterization of C3G: an exchange factor that discriminates between Rap1 and Rap2 and is not inhibited by Rap1A(S17N) Oncogene. ,vol. 15, pp. 845- 850 ,(1997) , 10.1038/SJ.ONC.1201407
Marta Rosário, Hugh F Paterson, Christopher J Marshall, Activation of the Raf/MAP kinase cascade by the Ras‐related protein TC21 is required for the TC21‐mediated transformation of NIH 3T3 cells The EMBO Journal. ,vol. 18, pp. 1270- 1279 ,(1999) , 10.1093/EMBOJ/18.5.1270