Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene.

摘要: The citrate synthase gene (gltA) of Bartonella henselae was cloned and sequenced to compare genetic divergence among alpha gamma branches the class Proteobacteria develop enhanced genotypic reagents for B. identification. gltA is 1,293 nucleotides in length 63 66% homologous with corresponding sequences Rickettsia prowazekii, Escherichia coli, Coxiella burnetii. observed variability suggests that can provide a useful means studying moderate related bacteria. Oligonucleotides specific were evaluated ability prime PCR amplification within proteobacteria. Under conditions used, only henselae, quintana, R. prowazekii template DNAs yielded products (approximately 380 bp). from 28 Bartonella-like isolates feline origin amplified by primers analyzed restriction fragment polymorphism. resulting patterns all similar or identical recognized strain. Current studies are aimed at optimization specificity sensitivity clinical isolates.