Phylogeographical diversity of Anaplasma phagocytophilum in the Asian part of Russia based on multilocus sequence typing and analysis of the ankA gene.

摘要: Anaplasma phagocytophilum is a tick-transmitted bacterium that replicates in neutrophil granulocytes and elicits febrile disease humans animals; it widely distributed the Americas, Europe, Africa, Asia. A. commonly regarded as single species, but several genetic variants with distinct host distribution geographical origin have been described. In previous study, we used multilocus sequence typing (MLST) to characterize 25 strains from Ixodes spp. ticks collected Asian part of Russia. The obtained concatenated sequences formed two separate clades reflecting their Asiatic and/or vector species. As one was related European voles shrews, here extended our analysis seven samples northern red-backed vole Myodes rutilus included 38 additional persulcatus, I. pavlovskyi, hybrids. Further, ankA gene sequenced 59 voles. Russian belonged new MLST clusters 5 (38/70) 6 (32/70), previously referred within 1 3, respectively. total number types (STs) found 27 including 12 STs. were unique clusters: cluster 8 (34/59) 10 (25/59). concordance between ankA-based 100%. This means at least groups circulate Part Russia whose reservoir hosts transmission cycles be further elucidated.