Insights into the chemical logic and enzymatic machinery of NRPS assembly lines.

摘要: Appreciation that some cyclic peptide antibiotics such as gramicidin S and tyrocidine were nonribosomally synthesized has been known for 50 years. The past two decades of research including advances in bacterial genetics, genomics, protein biochemistry mass spectrometry have codified the principles assembly line enzymology hundreds nonribosomal peptides parallel thousands polyketides. understanding strategies used chain initiation, elongation termination from these lines revitalized natural product biosynthetic communities.