Steroid hormone receptor expression and function in microglia

摘要: Steroid hormones such as glucocorticoids and estrogens are well-known regulators of peripheral immune responses also show anti-inflammatory properties in the brain. However, expression steroid hormone receptors microglia, pivotal cell that coordinates brain inflammatory response, is still controversial. Here we use real time RT-PCR to isolated from adult fms-EGFP mice by FACS, express glucocorticoid receptor (GR), mineralocorticoid (MR), estrogen alpha (ERalpha). GR was most abundant transcript microglia. The presence ERalpha immunoreactivity further confirmed vivo at ultrastructural level. To understand role during inflammation process, evaluated after challenge found a significant down-regulation GR, MR, Finally, tested immunomodulatory glucocorticoids. Estradiol benzoate did not have any impact on profile ex sorted either resting conditions or challenge. Furthermore, corticosterone more consistent agent than 17beta-estradiol vitro. Our results support hypothesis microglia direct target glucocorticoids, through predominant primary microglial activity. LPS may serve prerequisite suppressing actions endogenous system, contribute sustained activation