Investigation of the MALDI Process Used to Characterize Self-Assembled Monolayers of Alkanethiolates on Gold

作者: Tae Kyung Ha , Han Bin Oh , Jayong Chung , Tae Geol Lee , Sang Yun Han

DOI: 10.1021/LA8036567

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摘要: In this work, we investigated the surface processes involved in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), which produce intact characteristic ions, typically disulfide form, from self-assembled monolayers (SAMs) of alkanethiolates on gold. For study, SAMs decorated with peptides and a THAP matrix were employed. Using two-laser MS, it was previously found that irradiation UV gave rise to direct desorption SAM molecules alkanethiol gold, producing species vacuum. However, closer examination study suggests MALDI process is used may not be case. Instead, results indicate treatment solution responsible for ion formation MS. We propose dissolves alkanethiolate SAMs, leading generation solution. The disulfides are then cocrystallized ...

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