High throughput single-cell genome sequencing gives insights into the generation andevolution of mosaic aneuploidy in Leishmania donovani

摘要: Leishmania, a unicellular eukaryotic parasite, is unique model for aneuploidy and cellular heterogeneity, along with their potential role in adaptation to environmental stresses. Somy variation within clonal populations was previously explored small subset of chromosomes using fluorescence hybridization methods. This phenomenon, termed mosaic (MA), might have important evolutionary functional implications but remains under-explored due technological limitations. Here, we applied validated high throughput single-cell genome sequencing method study the first time extent dynamics whole karyotype heterogeneity two Leishmania representing different stages MA evolution vitro. We found that drastic changes karyotypes quickly emerge population stemming from an almost euploid founder cell. possibly involves polyploidization/hybridization at early stage expansion, followed by assorted ploidy reduction. During further increases moderate gradual karyotypic alterations. usually affected defined chromosomes, which some display enrichment snoRNA genes could represent adaptative benefit amplification these chromosomes. Our data provide complete characterization pave way studies.