The influence of glucocorticoids on the growth of a human leiomyosarcoma cell line SK-LMS-1

摘要: A cell line derived from a human leiomyosarcoma, SK-LMS-I, has cystolic [Bmax 201.6 +/- 39.7, means SD, fm/mg cytosol protein KD 14.6 7.8 nM (n = 8)] and nuclear 189.2 87.3 fM/mg DNA, 4.8 0.26 5)] glucocorticoid receptor, by Scatchard analysis of tumors grown in male athymic mice. Tumor growth SK-LMS-I cells mice is inhibited daily s.c. injection DEX 5 micrograms, 25 micrograms with mu RU-486 RU-486. In sharp contrast, vitro, markedly stimulates the (as determined number) cells, principally at higher densities (days 10-21 carried on over 21- to 23-day period), greatest stimulation being seen 10(-6) 10(-8) M, no 10(-9) 10(-10) M. glucocorticoids affinity for GR stimulate growth, steroids lower inhibit growth. No alterations cell-cycle distribution (percent G0/G1, S, or G2/m) could be found flow cytometric glucocorticoid-stimulated asynchronously growing cultures. Single, isolated, untreated form colonies soft agar an efficiency 1.78 0.10%. Pre-treatment 10(-7) M increases this 3.24 0.17%, while pre-treated both 10(7) same as cells. Glucocorticoids have inhibitory effects vivo stimulatory vitro GR-positive leiomyosarcoma line.