Differential Expression of Serum Proteins in Rats with Allergic Asthma: A Study Based on the Nanoliter Two-Dimensional Liquid Chromatography Technique
作者： Xingke Yan , Ao Zhang , Lu Yu , Cheng Chen , Haifu Cui
DOI: 10.1155/2017/8015703
关键词:
摘要: Objective. To investigate the pathogenesis of allergic asthma via nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique. Method. 24 Wistar rats were randomized into 3 groups: blank-control group (A), restrained (B), and model (C). The technique was used to study differential protein expressions serum in asthmatic rats. Results. Compared with group, data from displayed a distinctive peak, that is, peak number 13 (94.731 min). also three missing peaks 1 (77.489 min), 2 (78.418 min), 5 (80.533 min), suggesting corresponding peptides might be related asthma. showed 4 new peaks, (79.398 min), 7 (81.824 min). (78.804 min), indicating those polypeptides binding-induced stress stimuli. Conclusion. suggests is closely abnormal levels proteins; however, future animal experiments should identify specific caused by factors.
hindawi.com
core.ac.uk 参考文章(9)
Akira Okamoto, Tadao Hasegawa, Keiko Yamada, Michio Ohta, Application of both high-performance liquid chromatography combined with tandem mass spectrometry shotgun and 2-D polyacrylamide gel electrophoresis for streptococcal exoproteins gave reliable proteomic data Microbiology and Immunology. ,vol. 55, pp. 84- 94 ,(2011) , 10.1111/J.1348-0421.2010.00302.X
K E Wilson, Functional genomics and proteomics: application in neurosciences Journal of Neurology, Neurosurgery & Psychiatry. ,vol. 75, pp. 529- 538 ,(2004) , 10.1136/JNNP.2003.026260
Augusto M Tentori, Amy E Herr, Photopatterned materials in bioanalytical microfluidic technology. Journal of Micromechanics and Microengineering. ,vol. 21, pp. 054001- ,(2011) , 10.1088/0960-1317/21/5/054001
Valerie C. Wasinger, Stuart J. Cordwell, Anne Cerpa-Poljak, Jun X. Yan, Andrew A. Gooley, Marc R. Wilkins, Mark W. Duncan, Ray Harris, Keith L. Williams, Ian Humphery-Smith, Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium Electrophoresis. ,vol. 16, pp. 1090- 1094 ,(1995) , 10.1002/ELPS.11501601185
Seung-Ha Lee, TaiYoun Rhim, Yun-Sung Choi, Ji-Won Min, Sung-Ho Kim, Sun-Young Cho, Young-Ki Paik, Choon-Sik Park, Complement C3a and C4a Increased in Plasma of Patients with Aspirin-induced Asthma American Journal of Respiratory and Critical Care Medicine. ,vol. 173, pp. 370- 378 ,(2006) , 10.1164/RCCM.200505-740OC
Pierluigi Mauri, Anna Maria Riccio, Rossana Rossi, Dario Di Silvestre, Louise Benazzi, Laura De Ferrari, Roberto Walter Dal Negro, Stephen T. Holgate, Giorgio Walter Canonica, Proteomics of bronchial biopsies: Galectin-3 as a predictive biomarker of airway remodelling modulation in omalizumab-treated severe asthma patients Immunology Letters. ,vol. 162, pp. 2- 10 ,(2014) , 10.1016/J.IMLET.2014.08.010
J Craig Venter, Hamilton O Smith, Mark D Adams, The Sequence of the Human Genome Clinical Chemistry. ,vol. 61, pp. 1207- 1208 ,(2015) , 10.1373/CLINCHEM.2014.237016
Daniela Ferreira, Ana M.L. Seca, Diana C.G.A., Artur M.S. Silva, Targeting human pathogenic bacteria by siderophores: A proteomics review Journal of Proteomics. ,vol. 145, pp. 153- 166 ,(2016) , 10.1016/J.JPROT.2016.04.006
J Craig Venter, Mark D Adams, Eugene W Myers, Peter W Li, Richard J Mural, Granger G Sutton, Hamilton O Smith, Mark Yandell, Cheryl A Evans, Robert A Holt, Jeannine D Gocayne, Peter Amanatides, Richard M Ballew, Daniel H Huson, Jennifer Russo Wortman, Qing Zhang, Chinnappa D Kodira, Xiangqun H Zheng, Lin Chen, Marian Skupski, Gangadharan Subramanian, Paul D Thomas, Jinghui Zhang, George L Gabor Miklos, Catherine Nelson, Samuel Broder, Andrew G Clark, Joe Nadeau, Victor A McKusick, Norton Zinder, Arnold J Levine, Richard J Roberts, Mel Simon, Carolyn Slayman, Michael Hunkapiller, Randall Bolanos, Arthur Delcher, Ian Dew, Daniel Fasulo, Michael Flanigan, Liliana Florea, Aaron Halpern, Sridhar Hannenhalli, Saul Kravitz, Samuel Levy, Clark Mobarry, Knut Reinert, Karin Remington, Jane Abu-Threideh, Ellen Beasley, Kendra Biddick, Vivien Bonazzi, Rhonda Brandon, Michele Cargill, Ishwar Chandramouliswaran, Rosane Charlab, Kabir Chaturvedi, Zuoming Deng, Valentina Di Francesco, Patrick Dunn, Karen Eilbeck, Carlos Evangelista, Andrei E Gabrielian, Weiniu Gan, Wangmao Ge, Fangcheng Gong, Zhiping Gu, Ping Guan, Thomas J Heiman, Maureen E Higgins, Rui-Ru Ji, Zhaoxi Ke, Karen A Ketchum, Zhongwu Lai, Yiding Lei, Zhenya Li, Jiayin Li, Yong Liang, Xiaoying Lin, Fu Lu, Gennady V Merkulov, Natalia Milshina, Helen M Moore, Ashwinikumar K Naik, Vaibhav A Narayan, Beena Neelam, Deborah Nusskern, Douglas B Rusch, Steven Salzberg, Wei Shao, Bixiong Shue, Jingtao Sun, Zhen Yuan Wang, Aihui Wang, Xin Wang, Jian Wang, Ming-Hui Wei, Ron Wides, Chunlin Xiao, Chunhua Yan, Alison Yao, Jane Ye, Ming Zhan, Weiqing Zhang, Hongyu Zhang, Qi Zhao, Liansheng Zheng, Fei Zhong, Wenyan Zhong, Shiaoping C Zhu, Shaying Zhao, Dennis Gilbert, Suzanna Baumhueter, Gene Spier, Christine Carter, Anibal Cravchik, Trevor Woodage, Feroze Ali, Huijin An, Aderonke Awe, Danita Baldwin, Holly Baden, Mary Barnstead, Ian Barrow, Karen Beeson, Dana Busam, Amy Carver, Angela Center, Ming Lai Cheng, Liz Curry, Steve Danaher, Lionel Davenport, Raymond Desilets, Susanne Dietz, Kristina Dodson, Lisa Doup, Steven Ferriera, Neha Garg, Andres Gluecksmann, Brit Hart, Jason Haynes, Charles Haynes, Cheryl Heiner, Suzanne Hladun, Damon Hostin, Jarrett Houck, Timothy Howland, Chinyere Ibegwam, Jeffery Johnson, Francis Kalush, The Sequence of the Human Genome Science. ,vol. 291, pp. 1304- 1351 ,(2001) , 10.1126/SCIENCE.1058040